2024

Y.C. Hatasaki, R. Kobayashi, R.R. Watanabe, M. Hara, H. Ueno, and H. Noji
"Engineering of IF₁-susceptive bacterial F₁-ATPase"
(2024) Protein Science 33, e4942
published online

M. Sobti, H. Ueno, S.H.J. Brown, H. Noji*, and A.G. Stewart* (* corresponding authors)
"The series of conformational states adopted by rotorless F₁-ATPase during its hydrolysis cycle"
(2024) Structure 32, 1-7
published online

Y. Matsumoto, S. Yamasaki, K. Hayama, R. Iino, H. Noji, A. Yamaguchi, and K. Nishino
"Changes in the expression of mexB, mexY, and oprD in clinical Pseudomonas aeruginosa isolates"
(2024) Proceedings of the Japan Academy, Series B, 100, 57-67
published online

2023

H. Noji
"Rocking Out Biophysics in IUPAB2024 Kyoto!"
(2023) Biophysical and Physicobiology, 20, e200039
published online

H. Ueno, M. Sano, M. Hara, and H. Noji
"Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device"
(2023) ACS Sensors, 8, 3400-3407
published online

R. R. Watanabe, B. T. Kiper, M. Zarco-Zavala, M. Hara, R. Kobayashi, H. Ueno, J. J. García-Trejo, C. B. Li, and H. Noji
"Rotary properties of hybrid F₁-ATPases consisting of subunits from different species"
(2023) iScience, 26, 106626
published online

R. Kobayashi, H. Ueno, K. Okazaki, and H. Noji
"Molecular mechanism on forcible ejection of ATPase inhibitory factor 1 from mitochondrial ATP synthase"
(2023) Nat. Commun., 14, 1682
published online

T. Ono, KV. Tabata, H. Noji, J. Morimoto, and S. Sando
"Each side chain of cyclosporin A is not essential for high passive permeability across lipid bilayers"
(2023) RSC Adv., 13, 8394-8397
published online

M. Sakuma, S. Honda, H. Ueno, KV. Tabata, K. Miyazaki, N. Tokuriki*, and H. Noji* (* corresponding authors)
"Genetic Perturbation Alters Functional Substates in Alkaline Phosphatase"
(2023) J. Am. Chem. Soc., 145, 2806-2814
published online

T. Ono, KV. Tabata, Y. Goto, Y. Saito, H. Suga, H. Noji, J. Morimoto, and S. Sando
"Label-free quantification of passive membrane permeability of cyclic peptides across lipid bilayers: penetration speed of cyclosporin A across lipid bilayers"
(2023) Chemical Science, 14, 345-349
published online

Y. Minagawa, S. Nakata, M. Date, Y. Ii, and H. Noji
"On-Chip Enrichment System for Digital Bioassay Based on Aqueous Two-Phase System"
(2023) ACS Nano, 17, 212-220
published online

2022

CF. Wong, WG. Saw, S. Basak, M. Sano, H. Ueno, HW. Kerk, D. Litty, P. Ragunathan, T. Dick, V. Muller, H. Noji, and G. Gruber
"Structural Elements Involved in ATP Hydrolysis Inhibition and ATP Synthesis of Tuberculosis and Nontuberculous Mycobacterial F-ATP Synthase Decipher New Targets for Inhibitors"
(2022) Antimicrobial Agents and Chemotherapy, 66, e0105622
published online

K. Sato, R. Sasaki, R. Matsuda, M. Nakagawa, T. Ekimoto, T. Yamane, M. Ikeguchi, K. V. Tabata, H. Noji, and K. Kinbara
"Supramolecular Mechanosensitive Potassium Channel Formed by Fluorinated Amphiphilic Cyclophane"
(2022) J. Am. Chem. Soc., 144, 11802–11809
published online

H. Yaginuma, K. Ohtake, T. Akamatsu, H. Noji, and K. V. Tabata
"A microreactor sealing method using adhesive tape for digital bioassays"
(2022) Lab Chip, 22, 2001–2010
published online

Y. Itoh, S. Chen, R. Hirahara, T. Konda, T. Aoki, T. Ueda, I. Shimada, J. J. Cannon, C. Shao, J. Shiomi, K. V. Tabata, H. Noji, K. Sato, and T. Aida
"Ultrafast water permeation through nanochannels with a densely fluorous interior surface"
(2022) Science, 376, 738–743
published online

2021

M. Ugawa, Y. Kawamura, K. Toda, K. Teranishi, H. Morita, H. Adachi, R.Tamoto, H. Nomaru, K. Nakagawa, K. Sugimoto, E. Borisova, Y. An, Y. Konishi, S. Tabata, S. Morishita, M. Imai, T. Takaku, M. Araki, N. Komatsu, Y. Hayashi, I. Sato, R. Horisaki, H. Noji, and S. Ota
"In silico-labeled ghost cytometry"
(2021) Elife, 10, e67660
published online

H. Ueno, H. Sawada, N. Soga, M. Sano, S. Nara, K. V. Tabata, M. Su’etsugu, and H. Noji
"Amplification of over 100 kbp DNA from Single Template Molecules in Femtoliter Droplets"
(2021) ACS Synth. Biol., 10, 2179-2186
published online

M. Sobti, H. Ueno, H. Noji*, and A.G. Stewart*. (* corresponding authors)
"The six steps of the complete F₁-ATPase rotary catalytic cycle"
(2021) Nat Commun., 12, 4690
published online

H. Ueno, M. Kato, Y. Minagawa, Y. Hirose, and H. Noji
"Elucidation and control of low and high active populations of alkaline phosphatase molecules for quantitative digital bioassay"
(2021) Protein Sci., 30, 1628-1639
published online

S. Honda, Y. Minagawa, H. Noji, and K. V. Tabata
"Multidimensional Digital Bioassay Platform Based on an Air-Sealed Femtoliter Reactor Array Device"
(2021) Anal. Chem., 93, 5494–5502
published online

R. Kobayashi, S. Mori, H. Ueno, and H. Noji
"Kinetic analysis of the inhibition mechanism of bovine mitochondrial F₁-ATPase inhibitory protein using biochemical assay"
(2021) J. Biochem., 170, 79-87
published online

R. Sasaki, K. Sato, K. V. Tabata, H. Noji, and K. Kinbara
"Synthetic Ion Channel Formed by Multiblock Amphiphile with Anisotropic Dual-Stimuli-Responsiveness"
(2021) J. Am. Chem. Soc., 143 ,1348–1355
published online

2020

S. Yamaguchi, R. Takagi, T. Hosogane, Y. Ohashi, Y. Sakai, S. Sakakihara, R. Iino, K. V. Tabata, H. Noji, and A. Okamoto
"Single Cell Array Enclosed with a Photodegradable Hydrogel in Microwells for Image-Based Cell Classification and Selective Photorelease of Cells"
(2020) ACS Appl. Bio Mater., 3 , 5887–5895
published online

M. Zarco-Zavala, R. Watanabe, G. G. McMillan, T. Suzuki, H. Ueno, F. Mendoza-Hoffmann, J. J. García-Trejo, and H. Noji
"The 3 × 120° rotary mechanism of Paracoccus denitrificans F₁-ATPase is different from that of the bacterial and mitochondrial F1-ATPases"
(2020) Proc. Natl. Acad. Sci. USA, 117, 29647-29657
published online

T. Muraoka, D. Noguchi, R. S. Kasai, K. Sato, R. Sasaki, K. V. Tabata, T. Ekimoto, M. Ikeguchi, K. Kamagata,
No. Hoshino, H. Noji, T. Akutagawa, K. Ichimura, and K. Kinbara
"A synthetic ion channel with anisotropic ligand response"
(2020) Nature Communications, 11, 2924
published online

N. Soga, A. Ota, K. Nakajima, R. Watanabe, H. Ueno, and H. Noji
"Monodisperse Liposomes with Femtoliter Volume Enable Quantitative Digital Bioassays of Membrane Transporters and Cell-Free Gene Expression"
(2020) ACS Nano, 14, 11700-11711
published online

K. Akama, H. Noji
"Multiplexed homogeneous digital immunoassay based on single-particle motion analysis"
(2020) Lab Chip, 20, 2113-2121
published online

S. Sakamoto, T. Komatsu, R. Watanabe , Y. Zhang, T. Inoue, M. Kawaguchi, H. Nakagawa, T. Ueno, T. Okusaka, K. Honda, H. Noji, and Y. Urano
"Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins in biological samples"
(2020) Sci. Adv., 6, eaay0888
published online

R. Kobayashi, H. Ueno, C. Li, and H. Noji
"Rotary catalysis of bovine mitochondrial F₁-ATPase studied by single-molecule experiments"
(2020) Proc. Natl. Acad. Sci. USA, 117, 1447-81456
published online

2019

H. Ueno, Y. Kato, K. V. Tabata, and H. Noji
"Revealing the Metabolic Activity of Persisters in Mycobacteria by Single-Cell D2O Raman Imaging Spectroscopy"
(2019) Anal. Chem., 91, 15171-15178
published online

K. Akama, N. Iwanaga, K. Yamawaki, M. Okuda, K Jain, H. Ueno, N. Soga, Y. Minagawa and H. Noji
"Wash- and Amplification-Free Digital Immunoassay Based on Single-Particle Motion Analysis"
(2019) ACS Nano, 13, 13116-13126
published online

Y. Zhang, Y. Minagawa, H. Kizoe, K. Miyazaki, R. Iino, H. Ueno, K. V. Tabata, Y. Shimane and H. Noji
"Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array"
(2019) Sci. Adv., 5, eaav8185
published online

Y. Minagawa, H. Ueno, K.V. Tabata, and H. Noji
"Mobile imaging platform for digital influenza virus counting"
(2019) Lab Chip, 19, 2678-2687
published online

H. Eto, N. Soga, H.G. Franquelim, P. Glock, A. Khmelinskaia, L. Kai, M. Heymann, H. Noji, and P. Schwille
"Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer"
(2019) ACS Appl. Mater. Interfaces, 11, 21372-21380
published online

K.V. Tabata, T. Sogo, Y. Moriizumi, and H. Noji
"Regeneration of Escherichia coli Giant Protoplasts to Their Original Form"
(2019) Life, 9, 24
published online

Y. Moriizumi, K.V. Tabata, D. Miyoshi, and H. Noji
"Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System"
(2019) ACS Synth. Biol., 8, 557-567
published online

K.V. Tabata, Y. Minagawa, Y. Kawaguchi, M. Ono, Y. Moriizumi, S. Yamayoshi, Y. Fujioka, Y. Ohba, Y. Kawaoka, and H. Noji
"Antibody-free digital influenza virus counting based on neuraminidase activity"
(2019) Sci. Rep., 9, 1067
published online

2018

T. Ono, T. Ichiki, and H. Noji
"Digital enzyme assay using attoliter droplet array"
(2018) Analyst, 143, 4923-4929
published online

R. Watanabe, T. Komatsu, S. Sakamoto, Y. Urano, and H. Noji
"High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules"
(2018) Lab Chip, 18, 2849-2853
published online

Y. Moriizumi, K.V. Tabata, R. Watanabe, T. Doura, M. Kamiya, Y. Urano and H. Noji
"Hybrid cell reactor system from Escherichia coli protoplast cells and arrayed lipid bilayer chamber device"
(2018) Sci. Rep., 8, 11757
published online

H. Inoue, K. Kunida, N. Matsuda, D. Hoshino, T. Wada, H. Imamura, H. Noji, and S. Kuroda
"Automatic Quantitative Segmentation of Myotubes Reveals Single-cell Dynamics of S6 Kinase Activation"
(2018) Cell Struct Funct., 43, 2, 153-169
published online

S. Ota†, R. Horisaki†, Y. Kawamura†, M. Ugawa†, I. Sato, K. Hashimoto, R. Kamesawa, K. Setoyama, S Yamaguchi, K. Fujiu, K. Waki, H. Noji (†equal contributions)
"Ghost cytometry"
(2018) Science, 360, 1246-1251
published online

R. Watanabe, T. Sakuragi, H. Noji and S. Nagata
"Single-molecule analysis of phospholipid scrambling by TMEM16F"
(2018) Proc. Natl. Acad. Sci. USA, 115, 3066-3071
published online

A. Nakamura, T. Tasaki, Y. Okuni, C. Song, K. Murata, T. Kozai, M. Hara, H. Sugimoto, K. Suzuki, T. Watanabe, T. Uchihashi, H. Noji, and R. Iino
"Correction: Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis"
(2018) Phys Chem Chem Phys., 20, 3844
published online

K. Maeshima, T. Matsuda, Y. Shindo, H. Imamura, S. Tamura, R. Imai, S. Kawakami, R. Nagashima, T. Soga, H. Noji, K. Oka, and T. Nagai
"A Transient Rise in Free Mg²⁺ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation"
(2018) Curr Biol., 28,3, 444-451
published online

R. Watanabe, N. Soga, S. Ohdate and H. Noji
"Single-Molecule Analysis of Membrane Transporter Activity by Means of a Microsystem"
(2018) Bacterial Multidrug Exporters. Methods in Molecular Biology, 1700, 321-330
published online

R. Watanabe, M. Genda, Y. Kato-Yamada and H. Noji
"Essential Role of the ε Subunit for Reversible Chemo-Mechanical Coupling in F₁-ATPase"
(2018) Biophys. J., 114, 178-187
published online

Y. Tamiya, R. Watanabe, H. Noji, C. Li and T. Komatsuzaki
"Effects of non-equilibrium angle fluctuation on F₁-ATPase kinetics induced by temperature increase"
(2018) Phys. Chem. Chem. Phys., 20, 1872-1880
published online

2017

J. Li, G. He, H. Ueno, W. Liu, H Noji, C. Qi and X. Guo
"Direct measurement of single-molecule adenosine triphosphatase hydrolysis dynamics"
(2017) ACS Nano, 11, 12789-12795
published online

T. Muraoka, K. Umetsu, K.V. Tabata, T. Hamada, H. Noji, T. Yamashita and K. Kinbara
"Mechano-Sensitive Synthetic Ion Channels"
(2017) J. Am. Chem. Soc. 139, 18016–18023
published online

A. Nakamura, T. Tasaki, Y. Okuni, C. Song, K. Murata, T. Kozai, M. Hara, H. Sugimoto, K. Suzuki, T. Watanabe, T. Uchihashi, H. Noji and R. Iino
"Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis"
(2017) Phys. Chem. Chem. Phys., 20, 3010-3018
published online

2016

J. Li, G. He, H. Ueno, C. Jia, H. Noji, C. Qi and X. Guo
"Direct real-time detection of single proteins using silicon nanowire-based electrical circuits"
(2016) Nanoscale, 8, 16172-16176
published online

R. Watanabe, N. Soga, M. Hara and H. Noji
"Arrayed water-in-oil droplet bilayers for membrane transport analysis"
(2016) Lab Chip, 16, 3043-304
published online

M. Baba, K. Iwamoto, R. Iino, H. Ueno, M. Hara, A. Nakanishi, J. Kishikawa, H. Noji and K. Yokoyama
"Rotation of artificial rotor axles in rotary molecular motors"
(2016) Proc. Natl. Acad. Sci. USA, 113, 11214-11219
published online

D.G.G. McMillan, R. Watanabe, H. Ueno, G.M. Cook and H. Noji
"Biophysical Characterization of a Thermoalkaliphilic Molecular Motor with a High Stepping Torque Gives Insight into Evolutionary ATP Synthase Adaptation"
(2016) J. Biol. Chem., 291, 23965-23977
published online

H. Isojima, R. Iino, Y. Niitani, H. Noji and M. Tomishige
"Direct observation of intermediate states during the stepping motion of kinesin-1"
(2016) Nat. Chem. Biol., 12,290–297
published online

Y. Matsumoto, S. Sakakihara, A. Grushnikov, K. Kikuchi, H. Noji, A. Yamaguchi, R. Iino, Y. Yagi and K. Nishino
"A Microfluidic Channel Method for Rapid Drug-Susceptibility Testing of Pseudomonas aeruginosa"
(2016) Plos One, 11,e0148797
published online

2015

C. Li, H. Ueno, R. Watanabe, H. Noji and T. Komatsuzaki
"ATP hydrolysis assists phosphate release and promotes reaction ordering in F₁-ATPase"
(2015) Nat. Commun., 6, 1023
published online

R. Watanabe, N. Soga and H. Noji
"Novel Nano-Device to Measure Voltage-Driven Membrane Transporter Activity"
(2015) IEEE Transactions on Nanotechnology, 15, 70-73
published online

R. Hayashi, K. Sasaki, S. Nakamura, S. Kudo, Y. Inoue, H. Noji and K. Hayashi
"Giant Acceleration of diffusion observed in a single-molecule experiment on F₁-ATPase"
(2015) Phys. Rev. Lett., 114, 248101
published online

Y. Obayashi, R. Iino and H. Noji
"A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate"
(2015) Analyst, 140, 5065-5073
published online

N. Soga, R. Watanabe and H. Noji
"Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters"
(2015) Sci. Rep., 5, 11025
published online

H. Fujita, T. Esaki, T. Masujima, S.H. Kim, H. Noji and T. M. Watanabe
"Comprehensive chemical secretory measurement of single cell trapped in micro-droplet array with mass spectrometry"
(2015) RSC Adv., 5, 16968-16971
published online

S. Enoki, R. Iino, Y. Niitani, Y. Minagawa, M. Tomishige and H. Noji
"High-speed angle-resolved imaging of single gold nanorod with microsecond temporal resolution and one-degree angle precision"
(2015) Ana. Chem., 87, 2079-2086
published online

R. Watanabe, K. Koyasu, H. You, M. Tanigawara and H. Noji
"Torque transmission mechanism via DELSEED loop of F₁-ATPase"
(2015) Biophys. J., 108, 1144-1152
published online

2014

A. Yukawa, R. Iino, R. Watanabe, S. Hayashi and H. Noji
"Key Chemical Factors of Arginine Finger Catalysis of F₁-ATPase Clarified by an Unnatural Amino Acid Mutation"
(2014) Biochemistry, 54, 472-480
published online

R. Watanabe, N. Soga, T. Yamanaka and H. Noji
"High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition"
(2014) Sci. Rep., 4, 7076
published online

T. Muraoka, T. Endo, K.V. Tabata, H. Noji, S. Nagatoishi, K. Tsumoto, R. Li and K. Kinbara
"Reversible Ion Transportation Switch by a Ligand-Gated Synthetic Supramolecular Ion Channel"
(2014) J. Am. Chem. Soc., 136, 15584–15595
published online

M. Ui, K. Harima, T. Takei, K. Tsumoto, K.V. Tabata, H. Noji, S. Endo, K. Akiyama, T. Muraoka and K. Kinbara
"Grafting synthetic transmembrane units to the engineered low-toxicity α-hemolysin to restore its hemolytic activity"
(2014) Mol. BioSyst., 10, 3199-3206
published online

H. Ueno, Y. Minagawa, M. Hara, R. Suhaila, I. Yamato, E. Muneyuki, H. Noji, T. Murata and R. Iino
"Torque generation of Enterococcus hirae V-ATPase"
(2014) J. Biol. Chem., 289, 31212-31223
published online

R. Watanabe, Y. Minagawa and H. Noji
"Thermodynamic analysis of F₁-ATPase rotary catalysis using high-speed imaging"
(2014) Protein Sci., 23, 1773-1779
published online

H. Yaginuma, S. Kawai, K.V. Tabata, K. Tomiyama, A. Kakizuka, T. Komatsuzaki, H. Noji and H. Imamura
"Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging."
(2014) Sci. Rep., 4, 6522
published online

J. Kishikawa, A. Seino, A. Nakanishi, N.E. Tirtom, H. Noji, K. Yokokawa and K. Hayashi
"F-subunit reinforces torque generation in V-ATPase"
(2014) Eur. Biophys. J., 43, 415-422
published online

R. Watanabe†, N. Soga†, D. Fujita†, K.V. Tabata, L. Yamauchi, S.H. Kim, D. Asanuma, M. Kamiya, Y. Urano, H. Suga and H. Noji (†equal contributions)
"Arrayed Lipid Bilayer Chambers Allow Single-Molecule Analysis of Membrane Transporter Activity"
(2014) Nat. Commun., 5, 4519
published online

R. Watanabe, Y. Matsukage, A. Yukawa, K.V. Tabata and H. Noji
"Robustness of the Rotary Catalysis Mechanism of F₁-ATPase"
(2014) J. Biol. Chem., 289, 19331-19340
published online

T. Ikeda, T. Tsukahara, R. Iino, M. Takeuchi and H. Noji
"Motion Capture and Manipulation of Single Synthetic Molecular Rotors by Optical Microscopy"
(2014) Angew. Chem., 53, 10082-10085
published online 　

T. Ikeda, R. Iino and H. Noji
"Real-Time Fluorescence Visualization of Slow Tautomerization of Single Free-Base Phthalocyanines under Ambient Conditions"
(2014) Chem. Commun., 50, 9443-9446
published online 　

H.C. Arai, A. Yukawa, J.R. Iwatate, M. Kamiya, R. Watanabe, Y. Urano and H. Noji
"Torque generation mechanism of F₁-ATPase upon NTP binding"
(2014) Biophys. J., 107, 156-164
published online

T. Tanaka, K. Nagashima, N. Inagaki, H. Kioka, S. Takashima, H. Fukuoka, H. Noji, A. Kakizuka and H. Imamura
"Glucose-stimulated Single Pancreatic Islets Sustain Increased Cytosolic ATP Levels during Initial Ca2+ Influx and Subsequent Ca²⁺ Oscillations"
(2014) J. Biol. Chem., 289, 2205-2216
published online

R. Watanabe and H. Noji
"Characterization of the temperature-sensitive reaction of F₁-ATPase by using single molecule manipulation"
(2014) Sci. Rep., 4, 4962
published online

Y. Shibafuji, A. Nakamura, T. Uchihashi, N. Sugimoto, S. Fukuda, H. Watanabe, M. Samejima, T. Ando, H. Noji, A. Koivula, K. Igarashi and R. Iino
"Single-molecule imaging analysis of elementary reaction steps of Trichoderma Reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose I_α and III_I"
(2014) J. Biol. Chem., 289, 14056-14065
published online

R. Watanabe and H. Noji
"Timing of inorganic phosphate release modulates the catalytic activity of ATP-driven rotary motor protein"
(2014) Nat. Commun., 5, 3486
published online

2013

S.H. Kim, X. He, S. Kaneda, J. Kawada, D. Fourmy, H. Noji and T. Fujii
"Quantifying genetically inserted fluorescent protein in single iPS cells to monitor Nanog expression using electroactive microchamber arrays"
(2013) Lab Chip, 14, 730-736
published online

H. Kioka, H. Kato, M. Fujikawa, O. Tsukamoto, T. Suzuki, H. Imamura, A. Nakano, S. Higo, S. Yamazaki, T. Matsuzaki, K. Tkafuji, H. Asanuma, M. Asakura, T. Minamino, Y. Shintani, M. Yoshida, H. Noji, M. Kitakaze, I. Komuro, Y. Asano and S. Takashima
"Evaluation of intra-mitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation"
(2013) Proc. Natl. Acad. Sci. USA, 111, 273-278
published online

R. Watanabe, K. Hayashi, H. Ueno and H. Noji
"Catalysis-enhancement via rotary fluctuation of F₁-ATPase"
(2013) Biophys. J., 105, 2385-2391
published online

Y. Minagawa, H. Ueno, M. Hara, Y. Ishizuka-Katsura, N. Ohsawa, T. Terada, M. Shirouzu, S. Yokoyama, I. Yamato, E. Muneyuki, H. Noji, T. Murata and R. Iino
"Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V₁-ATPase"
(2013) J. Biol. Chem., 288, 32700-32707
published online

D. Okuno, M. Nishiyama and H. Noji
"Single molecule analysis of the rotation of F₁-ATPase under high hydrostatic pressure"
(2013) Biophys. J., 105, 1635-1642
published online

M. Toei and H. Noji
"Single-molecule analysis of F_oF₁-ATP synthase inhibited by N,N-dicyclohexylcarbodiimide"
(2013) J. Biol. Chem., 288, 25717-25726
published online

T. Tsuyama, J. Kishikawa, Y.W. Han, Y. Harada, A. Tsubouchi, H. Noji, A. Kakizuka, K. Yokoyama, T. Uemura and H. Imamura
"In vivo fluorescent ATP imaging of Drosophila melanogaster and Caenorhabditis elegans by using a genetically encoded fluorescent ATPbiosensor optimized for low temperatures"
(2013) Anal. Chem., 85, 7889-7896
published online

R. Watanabe, K.V. Tabata, R. Iino, H. Ueno, M. Iwamoto, S. Oiki, H. Noji
"Biased Brownian stepping rotation of F_oF₁-ATP synthase driven by proton motive force"
(2013) Nat. Commun., 4, 1631
published online

2012

N.E. Tirtom, D. Okuno, M. Nakano, K. Yokoyama and H. Noji
"Mechanical Modulation of ATP-Binding Affinity of V₁-ATPase"
(2012) J. Biol. Chem., 288, 619-623
published online

T. Muraoka, T. Shima, T. Hamada, M. Morita, M. Takagi, K.V. Tabata, H. Noji and K. Kinbara
"Ion Permeation by a Folded Multiblock Amphiphilic Oligomer Achieved by Hierarchical Construction of Self-Assembled Nanopores"
(2012) J. Am. Chem. Soc., 134, 19788-19794
published online

S. Enoki, R. Iino, N. Morone, K. Kaihatsu, S. Sakakihara, N. Kato and H. Noji
"Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field microscopy"
(2012) PLoS ONE, 7, e49208
published online

N. Hatsugai, V.P. Koldenkova, H. Imamura, H. Noji and T. Nagai
"Changes in Cytosolic ATP Levels and Intracellular Morphology during Bacteria-Induced Hypersensitive Cell Death as Revealed by Real-Time Fluorescence Microscopy Imaging"
(2012) Plant Cell Physiol., 53, 1768-1775
published online

S.H. Kim, S. Iwai, S. Araki, S. Sakakihara, R. Iino and H. Noji
"Large-scale femtoliter droplet array for digital counting of single biomolecules."
(2012) Lab Chip, 12, 4986-4991
published online

M. Tanigawara, K.V. Tabata, Y. Ito, J. Ito, R. Watanabe, H. Ueno, M. Ikeguchi and H. Noji
"Role of the DELSEED Loop in Torque Transmission of F₁-ATPase."
(2012) Biophys. J., 103, 970-978
published online

N.E. Tirtom, Y. Nishikawa, D. Okuno, M. Nakano, K. Yokoyama and H. Noji
"Single-molecule analysis of inhibitory pausing states of V₁-ATPase."
(2012) J. Biol. Chem., 287, 28327-28335
published online

H. You, R. Iino, R. Watanabe and H. Noji
"Winding single-molecule double-stranded DNA on a nanometer-sized reel."
(2012) Nucleic Acids Res., 40, e151
published online

R. Iino, K. Hayama, H. Amezawa, S. Sakakihara, S.H. Kim, Y. Matsumono, K. Nishino, A. Yamaguchi and H. Noji
"A single-cell drug efflux assay in bacteria by using a directly accessible femtoliter droplet array"
(2012) Lab Chip, 12, 3923-3929
published online

S. Hayashi, H. Ueno, A.R. Shaikh, M. Umemura, M. Kamiya, Y. Ito, M. Ikeguchi, Y. Komoriya, R. Iino and H. Noji
"Molecular mechanism of ATP hydrolysis in F₁-ATPase revealed by molecular simulations and single molecule observations"
(2012) J. Am. Chem. Soc., 134, 8447-8454
published online

Y. Komoriya, T. Ariga, R. Iino and H. Noji
"Principle role of the arginine finger in rotary catalysis of F₁-ATPase"
(2012) J. Biol. Chem., 287, 15134-15142
published online

K. Sasagawa, K. Ando, T. Kobayashi, T. Noda, T. Tokuda, S.H. Kim, R. Iino, H. Noji and J. Ohta
"Complementary Metal–Oxide–Semiconductor Image Sensor with Microchamber Array for Fluorescent Bead Counting"
(2012) Jpn. J. Appl. Phys., 51, 02BL01
published online 　

2011

R. Watanabe, D. Okuno, S. Sakakihara, K. Shimabukuro, R. Iino, M. Yoshida and H. Noji
"Mechanical modulation of catalytic power on F₁-ATPase"
(2011) Nat. Chem. Biol., 8, 86-92
published online 　

J. Kishikawa, M. Fujikawa, H. Imamura, K. Yasuda, H. Noji, N. Ishii, S. Mitani and K. Yokoyama
"Expression of ATP sensor protein in Caenorhabditis elegans"
(2011) Microscopy Research and Technique 2011
published online

T. Uchihashi, R, Iino, T. Ando and H. Noji
"High-speed atomic force microscopy reveals rotary catalysis of rotorless F₁-ATPase"
(2011) Science, 333, 755-758
published online 　

S. Furuike, M. Nakano, K. Adachi, H. Noji, K. Kinosita Jr. and K. Yokoyama
"Resolving stepping rotation in Thermus thermophilus H⁺-ATPase/synthase with an essentially drag-free probe"
(2011) Nat. Commun., 2, 233
published online

M. Nakano, H. Imamura, T. Nagai and H. Noji
"Ca²⁺ regulation of mitochondrial ATP synthesis visualized at the single cell level"
(2011) ACS Chem. Biol., 6, 709-715
published online

Y. Matsumoto, K. Hayama, S. Sakakihara, K. Nishino, H. Noji, R. Iino and A. Yamaguchi
"Evaluation of multidrug efflux pump inhibitors by a new method using microfluidic channels"
(2011) PLoS ONE, 6, e18547
published online

2010

S. Sakakihara, S. Araki, R. Iino and H. Noji
"A single-molecule enzymatic assay in a directly accessible femtoliter droplet array"
(2010) Lab Chip, 10, 3355-3362
published online

R. Watanabe, R. Iino and H. Noji
"Phosphate-release in F₁-ATPase catalytic cycle follows ADP release"
(2010) Nat. Chem Biol., 6, 814-820
published online 　

D. Okuno, R. Iino, and H. Noji
"Stiffness of γ subunit of F₁-ATPase"
(2010) Eur. Biophys. J., 39, 1589-1596
published oneline

K. Hayashi, H. Ueno, R. Iino and H. Noji
"Fluctuation theorem applied to F₁-ATPase"
(2010) Phys. Rev. Lett., 104, 218103
published online

H. Ueno, S. Nishikawa, R. Iino, K.V. Tabata, S. Sakakihara, T. Yanagida and H. Noji
"Simple Dark-Field Microscopy with Nanometer Spatial Precision and Microsecond Temporal Resolution"
(2010) Biophys. J., 98, 2014-2023
published online

T. Ide, Y. Takeuchi, H. Noji and K.V. Tabata
"Simultaneous Optical and Electrical Single Channel Recordings on a PEG Glass"
(2010) Langmuir, 26, 8540-8543
published online

I. Kotera, T. Iwasaki, H. Imamura, H. Noji and T. Nagai
"Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators"
(2010) ACS Chem. Biol., 5, 215-222
published online

2009

S.W. Lee, H. Kinoshita, H. Noji, T. Fujii and T. Yamamoto
"Biomolecular Nano-Flow-Sensor to Measure Near-Surface Flow"
(2009) Nanoscale Res. Lett., 5, 296–301
published online

T. Nakayama, M. Namura, K.V. Tabata, H. Noji and R. Yokokawa
"Sequential processing from cell lysis to protein assay on a chip enabling the optimization of an F₁-ATPase single molecule assay condition"
(2009) Lab Chip, 9, 3567-3573
published online

Y. Iko, K.V. Tabata, S. Sakakihara, T. Nakashima and H. Noji
"Acceleration of the ATP-binding rate of F₁-ATPase by forcible forward rotation."
(2009) FEBS Lett., 583, 3187-3191
published online

K.V. Tabata, K. Sato, T. Ide, T. Nishizaka, A. Nakano and H. Noji
"Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane"
(2009) EMBO J., 28, 3279-3289
published online

H. Imamura, K.P. Huynh Nhat, H. Togawa, K. Saito, R. Iino, Y. Kato-Yamada, T. Nagai and H. Noji
"Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators."
(2009) Proc. Natl. Acad. Sci. USA, 106, 15651-15656
PDF

S. Enoki, R. Watanabe, R. Iino and H. Noji
"Single-molecule study on the temperature-sensitive reaction of F₁-ATPase with a hybrid F1 carrying a single β(E190D)"
(2009) J. Biol. Chem., 284, 23169-13176
published online

Y. Hirono-Hara, H. Noji and S. Takeuchi
"Single-biomolecule observation with micro one-way valves for rapid buffer exchange."
(2009) J. Appl. Phys., 105, 102016
published online

R. Iino, R. Hasegawa, K.V. Tabata and H. Noji
"Mechanism of inhibition by C-terminal α-helices of the ε subunit of Escherichia coli F_oF₁-ATP synthase."
(2009) J. Biol. Chem., 284, 17457-17464
published online

2008

D. Okuno, R. Fujisawa, R. Iino, Y. Hirono-Hara, H. Imamura and H. Noji
"Correlation between the conformational states of F₁-ATPase as determined from its crystal structure and single-molecule rotation."
(2008) Proc. Natl. Acad. Sci. USA, 105, 20722-20727
PDF

S. Matsumoto, S. Yamaguchi, S. Ueno, H. Komatsu, M. Ikeda, K. Ishizuka, Y. Iko, K.V. Tabata, H. Aoki, S. Ito, H. Noji and I. Hamachi
"Photo Gel-Sol/Sol-Gel Transition and Its Patterning of a Supramolecular Hydrogel as Stimuli-Responsive Biomaterials."
(2008) Chem. Eur. J., 14, 3977-398
PDF

L. Lam, S. Sakakihara, K. Ishizuka, S. Takeuchi, H.F. Arata, H. Fujita and H. Noji
"Loop-mediated isothermal amplification of a single DNA molecule in polyacrylamide gel-based microchamber."
(2008) Biomed. Microdevices., 10, 539-546
PDF

R. Watanabe, R. Iino, K. Shimabukuro, M. Yoshida and H. Noji
"Temperature-sensitive reaction intermediate of F1-ATPase."
(2008) EMBO rep., 9, 84-90
PDF

S. Yamaguchi, S. Matsumoto, K. Ishizuka, Y. Iko, K.V. Tabata, H.F. Arata, H. Fujita, H. Noji and I. Hamachi
"Thermally Responsive Supramolecular Nanomeshes for On/Off Switching of the Rotary Motion of F1-ATPase at the Single-Molecule Level."
(2008) Chem. Eur. J., 14, 1891-1896
PDF

B. Le Pioufle, H. Suzuki, K.V. Tabata, H. Noji and S. Takeuchi
"Lipid bilayer microarray for parallel recording of transmembrane ion currents."
(2008) Anal. Chem., 80, 328-832
published online

2007

L. Lam, S. Sakakihara, K. Ishizuka, S. Takeuchi and H. Noji
"An integrated system for enzymatic cleavage and electrostretching of freely-suspended single DNA molecules."
(2007) Lab Chip, 7, 1738-1745
PDF

K. Adachi, K. Oiwa, T. Nishizaka, S. Furuike, H. Noji, H. Itoh, M. Yoshida, K. Kinosita Jr.
"Coupling of Rotation and Catalysis in F1-ATPase Revealed by Single-Molecule Imaging and Manipulation"
(2007) Cell, 130, 309-321
PDF

T. Nishinaka, Y. Doi, M. Hashimoto,R. Hara, T. Shibata, Y. Harada, K. KinositaJr, H. Noji and E. Yashima
"Visualization of RecA filaments and DNA by fluorescence microscopy."
(2007) J. Biochem. (Tokyo), 141, 147-156
PDF

2006

E. Muneyuki, T. Watanabe-Nakayama, T. Suzuki, M. Yoshida, T. Nishizaka and H. Noji
"Single Molecule Energetics of F1-ATPase motor."
(2006) Biophys. J., 92, 1806-1812
PDF

H.F. Arata, P. Low, K. Ishizuka, C. Bergaud, B. Kim, H. Noji and H. Fujita
"Temperature distribution measurement on microfabricated thermodevice for single biomolecular observation using fluorescent dye."
(2006) Sens. Actuators B-Chem., 117, 339-345
published online

S.W. Lee, T. Yamamoto, H. Noji and T. Fujii
"Chemical delivery microsystem for single-molecule analysis using multilaminar continuous flow."
(2006) Enzyme and Microb. Technol., 39, 519-525
published online

H.F. Arata, H. Noji and H. Fujita
"Motion control of single F1-ATPase rotary biomolecular motor using microfabricated local heating devices."
(2006) Appl. Phys. Lett., 88, 083902
published online

H. Suzuki, K.V. Tabata, H. Noji and S. Takeuchi
"Highly Reproducible Method of Planar Lipid Bilayer Reconstitution in Polymethyl Methacrylate Microfluidic Chip."
(2006) Langmuir, 22, 1937-1942
PDF

2005

A.R. Laine, D. Okuno, K.V. Tabata, Y. Okada, A. Tixier-Mita, H. Noji and H. Fujita
"Gold functionalized nano-needles for angular protein movement visualization."
(2005) Nanobiotechnology, 1, 227-236
PDF

H.F. Arata, Y. Rondelez, H. Noji and H. Fujita
"Temperature alternation by an on-chip microheater to reveal enzymatic activity of beta-galactosidase at high temperatures."
(2005) Anal. Chem., 77, 4810-4814
PDF

Y. Hirono-Hara, K. Ishizuka, K. Kinosita Jr., M. Yoshida and H. Noji
"Activation of pausing F1 motor by external force."
(2005) Proc. Natl. Acad. Sci. USA, 102, 4288-4293
PDF

Y. Rondelez, G. Tresset, T. Nakashima, Y. Kato-Yamada, H. Fujita, S. Takeuchi and H. Noji
"Highly coupled ATP synthesis by F1-ATPase single molecules."
(2005) Nature, 433, 773-777
PDF

Y. Rondelez, G. Tresset, K.V. Tabata, H. Arata, H. Fujita, S. Takeuchi and H. Noji
"Microfabricated arrays of femtoliter chambers allow single molecule enzymology"
(2005) Nat. Biotech., 3, 361-365
PDF

H. Noji, Y. Rondelez, T. Nakashima, G. Tresset, K.V. Tabata, Y. Kato-Yamada, H. Fujita and S. Takeuchi
"Ultra-small chamber for single-molecule detection of biological reaction"
(2005) e-J. Surf. Sci. Nanotech, 3, 79-81
published online

T. Ide, Y. Takeuchi, T. Aoki, K.V. Tabata and H. Noji
"An artificial lipid bilayer formed on a PEG-coated glass for simultaneous electrical and optical measurement of single ion-channels."
(2005) e-J. Surf. Sci. Nanotech, 3, 70-73
published online

2004

H. Suzuki H., K.V. Tabata, Y. Kato-Yamada, H. Noji and S. Takeuchi
"Planar lipid bilayer reconstitution with a micro-fluidic system"
(2004) Lab chip, 4, 502-505
published online

K. Atsuta, H. Noji and S. Takeuchi
"Micro patterning of active proteins with perforated PDMS sheets (PDMS sieve)"
(2004) Lab Chip, 4, 333-336
published online

H. Itoh, A. Takahashi, K. Adachi, H. Noji, R. Yasuda, M. Yoshida and K. Kinosita Jr.
"Mechanically driven ATP synthesis by F₁-ATPase."
(2004) Nature, 427, 465-468
published online

T. Nishizaka, K. Oiwa, H. Noji, S. Kimura, E. Muneyuki, M. Yoshida and K. KinositaJr.
"Chemomechanical coupling in F₁-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation."
(2004) Nat. Struct. Mol. Biol., 11, 142-148
published online

2003

S. Ono, K.Y. Hara, J. Hirao, T. Matsui, H. Noji, M. Yoshida and E. Muneyuki
"Origin of apparent negative cooperativity of F₁-ATPase."
(2004) Biochim. Biophys. Acta., 1607, 35-44
published online

R. Yasuda, T. Masaike, K. Adachi, H. Noji and K. Kinosita Jr.
"The ATP-waiting conformation of rotating F₁-ATPase revealed by single-pair fluorescence resonance energy transfer."
(2004) Proc. Natl. Acad. Sci. USA, 100, 9314-9318
published online

H. Imamura, M. Nakano, H. Noji, E. Muneyuki, S. Ohkuma, M. Yoshida and K. Yokoyama
"Evidence for rotation of V₁-ATPase"
(2004) Proc. Natl. Acad. Sci. USA, 100, 2312-2315
published online

2002

T. Ariga, T. Masaike, H. Noji and M. Yoshida
"Stepping rotation of F₁-ATPase with 1, 2, or 3 altered catalytic sites that bind ATP only slowly."
(2002) J. Biol. Chem., 277, 24870-24874
published online

T. Masaike, E. Muneyuki, H. Noji, K. Kinosita Jr. and M. Yoshida
"F₁-ATPase changes its conformations upon phosphate release."
(2002) J. Biol. Chem., 277, 21643-21649
published online

2001

Y. Hirono-Hara, H. Noji, M. Nishiura, E. Muneyuki, K.Y. Hara, R.Yasuda, K. Kinosita Jr. and M. Yoshida
"Pause and rotation of F₁-ATPase during catalysis."
(2002) Proc. Natl. Acad. Sci. USA, 98, 13649-13654
published online

D. Bald, H. Noji, M. Yoshida, Y. Hirono-Hara and T. Hisabori
"Redox regulation of the rotation of F₁-ATP synthase."
(2002) J. Biol. Chem., 276, 39505-7
published online

R. Yasuda, H. Noji, M. Yoshida, K. Kinosita Jr. and H. Itoh
"Resolution of distinct rotational substeps by submillisecond kinetic analysis of F₁-ATPase."
(2002) Nature, 410, 898-904
published online -->

H. Noji, D. Bald, R. Yasuda, H. Itoh, M. Yoshida and K. Kinosita Jr.
"Purine but not pyrimidine nucleotides support rotation of F₁-ATPase."
(2002) J. Biol. Chem., 276, 25480-25486
published online

2000

K. Adachi, R. Yasuda, H. Noji, H. Itoh, Y. Harada, M. Yoshida and K. Kinosita Jr.
"Stepping rotation of F₁-ATPase visualized through angle-resolved single-fluorophore imaging."
(2000) Proc. Natl. Acad. Sci. USA, 97, 7243-7247
publised online

K.Y. Hara, H. Noji, D. Bald, R. Yasuda, K. Kinosita Jr. and M. Yoshida
"The role of the DELSEED motif of the β subunit in rotation of F₁-ATPase."
(2000) J. Biol. Chem., 275, 14260-14263
published online

D. Bald and H. Noji, M.T. Stumpp, M. Yoshida and T. Hisabori
"ATPase activity of a highly stable 33 subcomplex of thermophilic F₁ can be regulated by the introduced regulatory region of γ subunit of chloroplast F₁."
(2000) J. Biol. Chem., 275, 12757-12762
published online

S.P. Tsunoda, R. Aggeler, H. Noji, K. Kinosita Jr., M. Yoshida and R.A. Capaldi
"Observations of rotation within the F_oF₁-ATP synthase: deciding between rotation of the Foc subunit ring and artifact."
(2000) FEBS Lett., 470, 244-248
published online -->

T. Masaike, N. Mitome, H. Noji, E. Muneyuki, R. Yasuda, K. Kinosita Jr. and M. Yoshida
"Rotation of F₁-ATPase and the hinge residues of the β subunit."
(2000) J. Exp. Biol., 203, 1-8
< published online

1999

T. Amano, T. Matsui, E. Muneyuki, H. Noji, K. Hara, M. Yoshida and T. Hisabori
"α₃β₃γ complex of F₁-ATPase from thermophilic Bacillus PS3 can maintain steady-state ATP hydrolysis activity depending on the number of non-catalytic sites."
(1999) Biochem J., 343, 135-8.
published online

H. Noji, K. Hasler, W. Junge, K. Kinosita Jr., M. Yoshida and S. Engelbrecht
"Rotation of Escherichia coli F₁-ATPase."
(1999) Biochem. Biophys. Res. Commun., 260, 597-599
published online

S.P. Tsunoda, E. Muneyuki, T. Amano, M. Yoshida and H. Noji
"Cross-linking of two β subunits in the closed conformation in F₁-ATPase."
(1999) J. Biol. Chem., 274, 5701-5706
published online

1998

Y. Kato-Yamada, H. Noji, R. Yasuda, K. Kinosita Jr. and M. Yoshida
"Direct observation of the rotation of ε subunit in F₁-ATPase."
(1998) J. Biol. Chem., 273, 19375-19377
published online

R. Yasuda, H. Noji, K. Kinosita Jr. and M. Yoshida
"F₁-ATPase is a highly efficient molecular motor that rotates with discrete 120 degree steps."
(1998) Cell, 93, 1117-1124
published online

1997

H. Noji, R. Yasuda, M. Yoshida and K. Kinosita Jr.
"Direct observation of the rotation of F₁-ATPase."
(1997) Nature, 386, 299-302
published online

2021

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「生物物理学の展開「はかる」から「つくる」学問へ」
現代科学 2021年3月号 創刊50周年記念号, p38-41, 2021年3月

2020

高久春雄、野地博行
「細胞創生のための合成ゲノムとその応用」
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現代科学増刊 「相分離生物学の全貌」, p334-337, 2020年11月